RCF RPM Calculator

RCF Formula:

\[ RCF = 1.118 \times 10^{-5} \times r \times RPM^2 \]

Relative Centrifugal Force (RCF):

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1. What is RCF?

Relative Centrifugal Force (RCF) is the measurement of the force applied to a sample within a centrifuge. It is expressed in multiples of the Earth's gravitational force (g) and provides a standardized way to compare centrifugation protocols across different equipment.

2. How Does the Calculator Work?

The calculator uses the RCF formula:

\[ RCF = 1.118 \times 10^{-5} \times r \times RPM^2 \]

Where:

\( RCF \) — Relative centrifugal force (g)
\( r \) — Radius from center of rotation to sample (cm)
\( RPM \) — Rotations per minute (rev/min)
\( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The formula converts rotational speed (RPM) to gravitational force equivalents, accounting for the radius of rotation.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is essential for reproducible centrifugation protocols in laboratory settings. Using RCF instead of RPM ensures consistent results across different centrifuges with varying rotor sizes.

4. Using the Calculator

Tips: Enter the radius in centimeters (measured from the center of rotation to the sample) and the RPM value. Both values must be positive numbers greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of RPM?
A: RCF provides a standardized measure of centrifugal force that accounts for rotor radius, making protocols reproducible across different centrifuges.

Q2: How do I measure the radius accurately?
A: Measure from the center of the centrifuge rotor to the middle of the sample tube when it's in the horizontal position during rotation.

Q3: What are typical RCF values used in laboratories?
A: RCF values range from 100-20,000 g depending on the application, with lower forces for cell separation and higher forces for pelleting small particles.

Q4: Can I convert RCF back to RPM?
A: Yes, the formula can be rearranged: \( RPM = \sqrt{\frac{RCF}{1.118 \times 10^{-5} \times r}} \)

Q5: Does temperature affect RCF calculation?
A: No, RCF calculation is purely based on rotational mechanics and is not affected by temperature, though temperature may affect sample behavior during centrifugation.