RCF to RPM Calculator

RPM Formula:

\[ RPM = \sqrt{ \frac{RCF}{1.118 \times 10^{-5} \times r} } \]

RPM (rev/min):

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1. What is RCF to RPM Conversion?

The RCF (Relative Centrifugal Force) to RPM (Revolutions Per Minute) conversion calculates the rotational speed needed to achieve a specific centrifugal force in a centrifuge. This is essential for laboratory procedures requiring precise separation of samples.

2. How Does the Calculator Work?

The calculator uses the RPM formula:

\[ RPM = \sqrt{ \frac{RCF}{1.118 \times 10^{-5} \times r} } \]

Where:

\( RCF \) — Relative Centrifugal Force (g)
\( r \) — Radius from center to sample (cm)
\( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The formula converts gravitational force (RCF) to rotational speed (RPM) based on the centrifuge's radius, allowing precise control over separation conditions.

3. Importance of RPM Calculation

Details: Accurate RPM calculation ensures consistent and reproducible results in centrifugation processes, critical for cell separation, protein purification, and other laboratory applications.

4. Using the Calculator

Tips: Enter RCF value in g units and radius in centimeters. Both values must be positive numbers. The calculator will compute the required RPM for your centrifuge.

5. Frequently Asked Questions (FAQ)

Q1: What is the difference between RCF and RPM?
A: RCF measures the actual gravitational force applied to samples, while RPM measures the rotational speed. RCF is more meaningful for comparing centrifugation results across different centrifuges.

Q2: Why is radius important in this calculation?
A: The centrifugal force increases with radius at the same RPM. Larger radius centrifuges generate higher RCF at the same rotational speed.

Q3: How do I measure the radius accurately?
A: Measure from the center of the rotor to the middle of the sample tube when it's in the horizontal position during rotation.

Q4: Can I use this calculator for any type of centrifuge?
A: Yes, the formula applies to all centrifuges, but ensure you're using the correct radius measurement for your specific rotor and tube position.

Q5: What are typical RCF values used in laboratories?
A: Typical ranges vary: 200-500g for cell pelleting, 2,000-5,000g for protein precipitation, and up to 100,000g for ultracentrifugation of subcellular components.