RPM To RCF Calculator Sorvall

Sorvall Centrifuge RCF Equation:

\[ RCF = 1.118 \times 10^{-5} \times r \times RPM^2 \]

RCF (g):

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1. What Is The RPM To RCF Calculator Sorvall?

The RPM to RCF Calculator for Sorvall centrifuges converts rotational speed (RPM) to relative centrifugal force (RCF or g-force) based on the rotor radius. This conversion is essential for standardizing centrifugation protocols across different centrifuge models.

2. How Does The Calculator Work?

The calculator uses the Sorvall centrifuge RCF equation:

\[ RCF = 1.118 \times 10^{-5} \times r \times RPM^2 \]

Where:

\( RCF \) — Relative Centrifugal Force (g)
\( r \) — Rotor radius (cm)
\( RPM \) — Rotations per minute (rev/min)
\( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The equation calculates the gravitational force experienced by samples during centrifugation, which depends on both rotational speed and rotor radius.

3. Importance Of RCF Calculation

Details: Accurate RCF calculation ensures reproducible experimental results across different centrifuges and protocols. RCF (g-force) is the standard parameter for centrifugation methods, not RPM, as different rotors with the same RPM produce different forces.

4. Using The Calculator

Tips: Enter RPM in revolutions per minute and rotor radius in centimeters. Ensure accurate radius measurement from the center of rotation to the sample position. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why Use RCF Instead Of RPM?
A: RCF provides consistent results across different centrifuge models and rotor sizes, while the same RPM produces different forces in different rotors.

Q2: How Do I Measure Rotor Radius?
A: Measure from the center of the rotor to the middle of the sample tube when placed in the rotor. Refer to your centrifuge manual for specific radius values.

Q3: What Are Typical RCF Values For Common Protocols?
A: Cell pelleting: 200-500g, nucleic acid precipitation: 10,000-15,000g, protein precipitation: 15,000-20,000g, ultracentrifugation: 100,000g+.

Q4: Does Temperature Affect RCF Calculation?
A: No, RCF calculation is independent of temperature. However, temperature control is important for sample integrity during centrifugation.

Q5: Can I Use This Calculator For Other Centrifuge Brands?
A: Yes, the RCF calculation formula is universal for all centrifuges. The equation applies to any centrifuge when you know the rotor radius.