1. What is Relative Centrifugal Force?

Relative Centrifugal Force (RCF) is the measurement of the force applied to a sample within a centrifuge. It is expressed in multiples of the earth's gravitational force (g) and provides a standardized way to compare centrifugation protocols across different equipment.

2. How Does the Calculator Work?

The calculator uses the RCF formula:

Where:

• \( RCF \) — Relative centrifugal force (g)
• \( r \) — Radius from center of rotation to sample (cm)
• \( RPM \) — Rotations per minute (rev/min)
• \( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The formula converts rotational speed (RPM) to the actual gravitational force experienced by samples, accounting for the centrifuge's radius.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is essential for reproducible centrifugation protocols, ensuring consistent separation results across different centrifuges and laboratories.

4. Using the Calculator

Tips: Enter the radius in centimeters (measured from center to sample) and RPM value. Both values must be positive numbers greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of RPM?
A: RCF provides a standardized measurement that accounts for different centrifuge sizes, ensuring consistent results regardless of equipment used.

Q2: How do I measure the radius accurately?
A: Measure from the center of the rotor to the middle of the sample tube when it's in the spinning position.

Q3: What are typical RCF values used in laboratories?
A: RCF values range from 100-20,000g depending on the application, with lower forces for cell separation and higher forces for pelleting small particles.

Q4: Can I use this calculator for any type of centrifuge?
A: Yes, the formula applies to all types of centrifuges as long as you use the correct radius measurement.

Q5: What's the difference between RCF and RPM?
A: RPM measures rotational speed, while RCF measures the actual force applied to samples. Two centrifuges with same RPM but different radii will produce different RCF values.