RPM to RCF Formula

RCF Formula:

\[ RCF = RPM² \times r \times 1.118 \times 10^{-5} \]

RCF (g-force):

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1. What is the RPM to RCF Formula?

The RPM to RCF (Relative Centrifugal Force) formula converts rotational speed in revolutions per minute (RPM) to g-force, taking into account the radius of the centrifuge rotor. This is essential for standardizing centrifugation protocols across different equipment.

2. How Does the Calculator Work?

The calculator uses the RCF formula:

\[ RCF = RPM² \times r \times 1.118 \times 10^{-5} \]

Where:

\( RCF \) — Relative centrifugal force (g-force)
\( RPM \) — Rotational speed (revolutions per minute)
\( r \) — Radius from center of rotation to sample (centimeters)
\( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The formula calculates the centrifugal force experienced by samples during centrifugation, which is proportional to the square of the rotational speed and the radius of rotation.

3. Importance of RCF Calculation

Details: Accurate RCF calculation ensures reproducible centrifugation results across different centrifuges and rotors. Using RPM alone can lead to inconsistent results due to varying rotor radii.

4. Using the Calculator

Tips: Enter RPM value in revolutions per minute and radius in centimeters. Both values must be positive numbers. The radius should be measured from the center of rotation to the sample position.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of RPM?
A: RCF provides a standardized measure of centrifugal force that accounts for rotor radius, ensuring consistent results across different centrifuges.

Q2: How do I measure the radius?
A: Measure from the center of the centrifuge rotor to the middle of the sample tube when it's in the horizontal position during rotation.

Q3: What is the typical range for RCF values?
A: RCF values typically range from 100 g for gentle separations to over 100,000 g for high-speed ultracentrifugation.

Q4: Can I use this formula for any centrifuge?
A: Yes, the formula is universal and applies to all centrifuges, but always verify the radius measurement for your specific rotor.

Q5: Why is the radius squared in the formula?
A: The centrifugal force increases with the square of the rotational speed, making higher RPM values exponentially more powerful.